Intermediate

Part:BBa_K648000:Design

Designed by: Lauren Rossi   Group: iGEM11_Penn_State   (2011-05-27)


mCherry with terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the n terminus of mCherry had a sequence which could act as a ribosome binding site near an internal methionine codon which could interfere with the the ribosomes affinity for the actual ribosome binding site. The first 8 amino acids were removed without having any effect on fluorescence.


Source

Parts registry

References